| Properties | Information | |
|---|---|---|
| PhytoCAT-ID | PhytoCAT-601 | |
| Phytochemical name or plant extracts | Sinomenine | |
| PMID | 30797069 | |
| Literature evidence | More interestingly, SIN was demonstrated to decrease hIL-8 mRNA expression in cultured MDA-MB-231 cells and to inhibit hIL-8 protein expression in MDA-MB-231 cells cocultured with preosteoclastic RAW264.7 cells while simultaneously downregulating CXCR1, the ligand of IL-8 related to bone destruction, during MDA-MB-231 CM-induced osteoclastogenesis. | |
| IUPAC name | (1R,9S,10S)-3-hydroxy-4,12-dimethoxy-17-methyl-17-azatetracyclo[7.5.3.01,10.02,7]heptadeca-2(7),3,5,11-tetraen-13-one | |
| Phytochemicals’ class or type of plant extracts | Alkaloid | |
| Source of phytochemicals or plant Extracts | Sinomenium acutum | |
| Geographical availability | China North-Central, China South-Central, China Southeast, Japan, Korea, Nansei-shoto, Nepal, Thailand | |
| Plant parts | NA | |
| Other cancers | Breast cancer | |
| Target gene or protein | TRAP, OSCAR, c-Fos, NFATc1, hIL-8, IL-8/CXCR1, MMP2, MMP9 | |
| Gene or Protein evidence | The MDA-MB-231 CM-induced osteoclast-related genes TRAP and OSCAR were obviously downregulated by SIN. In addition, mRNA expression of c-Fos and NFATc1 and nuclear translocation of c-Fos and NFATc1 protein were inhibited by SIN during MDA-MB-231 CM-induced osteoclastogenesis, while NF-kB signaling was not impacted by SIN. More interestingly, SIN was demonstrated to decrease hIL-8 mRNA expression in cultured MDA-MB-231 cells and to inhibit hIL-8 protein expression in MDA-MB-231 cells cocultured with preosteoclastic RAW264.7 cells while simultaneously downregulating CXCR1, the ligand of IL-8 related to bone destruction, during MDA-MB-231 CM-induced osteoclastogenesis. Our current findings may extend the utilization of SIN to preventing osteoclastogenesis and bone destruction in breast cancer patients and may enable IL-8/CXCR1 to serve as new targets for both anticancer and antiarthritic drug discovery. Western blot assay results showed that the upregulation of MMP-2 and MMP-9 by hypoxia was inversed by sinomenine. | |
| Target pathways | IL-8/CXCR1 and c-Fos/NFATc1 signaling PI3K/Akt/mTOR pathway | |
| IC50 | NA | |
| Potency | Sinomenine inhibits hypoxia induced breast cancer side population cells metastasis by PI3K/Akt/mTOR pathway. | |
| Cell line/ mice model | Tumor-bearing mice, MDA-MB-231 | |
| Additional information | Our current findings may extend the utilization of SIN to preventing osteoclastogenesis and bone destruction in breast cancer patients and may enable IL-8/CXCR1 to serve as new targets for both anticancer and antiarthritic drug discovery. | |
| PubChem ID | 5459308 | |
| Additional PMIDs | 25749075 33412412 34200341 | |
| Additional sources of information | https://powo.science.kew.org/taxon/urn:lsid:ipni.org:names:581373-1 | |
| Safety | NA |