| Properties | Information | |
|---|---|---|
| PhytoCAT-ID | PhytoCAT-2083 | |
| Phytochemical name or plant extracts | Silvestrol | |
| PMID | 26762417 | |
| Literature evidence | Silvestrol represents a natural product scaffold that exhibits potent cytotoxic activity and could be used for the further study of autophagy and its relationship to apoptosis in cancer cells. | |
| IUPAC name | methyl (1R,2R,3S,3aR,8bS)-6-[[(2S,3R,6R)-6-[(1R)-1,2-dihydroxyethyl]-3-methoxy-1,4-dioxan-2-yl]oxy]-1,8b-dihydroxy-8-methoxy-3a-(4-methoxyphenyl)-3-phenyl-2,3-dihydro-1H-cyclopenta[b][1]benzofuran-2-carboxylate | |
| Phytochemicals’ class or type of plant extracts | Benzofuran | |
| Source of phytochemicals or plant Extracts | Aglaia foveolata | |
| Geographical availability | Borneo, Malaya, Sumatera | |
| Plant parts | Fruits and twigs | |
| Other cancers | Breast cancer | |
| Target gene or protein | Cyclin B, Cyclin D | |
| Gene or Protein evidence | By 24 h, a decrease in cyclin B and cyclin D expression was observed in silvestrol-treated cells relative to control. | |
| Target pathways | NA | |
| IC50 | 1.6 nM against MDA-MB-435 | |
| Potency | Silvestrol potently inhibits cell growth and induces cell death in human melanoma cells through induction of early autophagy and caspase-mediated apoptosis. Silvestrol represents a natural product scaffold that exhibits potent cytotoxic activity and could be used for the further study of autophagy and its relationship to apoptosis in cancer cells. | |
| Cell line/ mice model | MDA-MB-435 | |
| Additional information | In addition, silvestrol blocked progression through the cell cycle at the G2-phase. In silvestrol-treated cells, DAPI staining of nuclear chromatin displayed nucleosomal fragments. Annexin V staining demonstrated an increase in apoptotic cells after silvestrol treatment. Silvestrol induced caspase-3 activation and apoptotic cell death in a time- and dose-dependent manner. Furthermore, both silvestrol and SAHA enhanced autophagosome formation in MDA-MB-435 cells. MDA-MB-435 cells responded to silvestrol treatment with accumulation of LC3-II and time-dependent p62 degradation. Bafilomycin A, an autophagy inhibitor, resulted in the accumulation of LC3 in cells treated with silvestrol. Silvestrol-mediated cell death was attenuated in ATG7-null mouse embryonic fibroblasts (MEFs) lacking a functional autophagy protein. | |
| PubChem ID | 11787114 | |
| Additional PMIDs | NA | |
| Additional sources of information | https://powo.science.kew.org/taxon/urn:lsid:ipni.org:names:968391-1 | |
| Safety | NA |