| Properties | Information | |
|---|---|---|
| PhytoCAT-ID | PhytoCAT-655 | |
| Phytochemical name or plant extracts | Pseudolaric acid B | |
| PMID | 33133258 | |
| Literature evidence | Additional experiments demonstrated that PAB regulated the activation of caspase-8 and caspase-9 to induce apoptosis and caused an upregulation of phosphorylated H2A histone family member X and cyclin B1 expression in order to induce cell cycle arrest. | |
| IUPAC name | (2E,4E)-5-[(1S,7S,8S,9R)-7-acetyloxy-4-methoxycarbonyl-9-methyl-11-oxo-10-oxatricyclo[6.3.2.01,7]tridec-3-en-9-yl]-2-methylpenta-2,4-dienoic acid | |
| Phytochemicals’ class or type of plant extracts | Diterpenoid-type acid | |
| Source of phytochemicals or plant Extracts | Pseudolarix kaempferi | |
| Geographical availability | Japan | |
| Plant parts | Root and stem | |
| Other cancers | Breast cancer | |
| Target gene or protein | HIF-1α, Caspase 8, Caspase 9, Cyclin B1 | |
| Gene or Protein evidence | PAB abrogated hypoxia-induced VEGF secretion from MDA-MB-468 cells via reducing HIF-1α protein. Additional experiments demonstrated that PAB regulated the activation of caspase-8 and caspase-9 to induce apoptosis and caused an upregulation of phosphorylated H2A histone family member X and cyclin B1 expression in order to induce cell cycle arrest. | |
| Target pathways | NA | |
| IC50 | 0.42 μmol/L against MDA-MB-468 for 72h | |
| Potency | PAB displays the dual antiangiogenic activities of directly inhibiting endothelial cells and abrogating paracrine stimulation of VEGF from tumor cells due to reducing HIF-1α protein by promoting its proteasome-mediated degradation in MDA-MB-468 cells, which has potential clinical relevance | |
| Cell line/ mice model | MDA-MB-468, MDA-MB-435, L929, MRC5, SW579, MCF-7 | |
| Additional information | Additional analyses using LY294002 and U0126 indicated that the increase in hypoxia-inducible factor 1 (HIF-1)α protein level was highly dependent on phosphatidylinositol 3′-kinase and p42/p44 mitogen-activated protein kinase activities in hypoxic MDA-MB-468 cells. However, PAB treatment did not affect the active (phosphorylated) forms of Akt and Erk. Interestingly, the selective proteasome inhibitor MG-132 completely reversed the reduction of HIF-1α protein in the PAB-treated MDA-MB-468 cells. | |
| PubChem ID | 71307573 | |
| Additional PMIDs | 15623602 | |
| Additional sources of information | https://powo.science.kew.org/taxon/urn:lsid:ipni.org:names:60457419-2 | |
| Safety | NA |