| Properties | Information | |
|---|---|---|
| PhytoCAT-ID | PhytoCAT-1919 | |
| Phytochemical name or plant extracts | Huaier extract | |
| PMID | 29145193 | |
| Literature evidence | Huaier Extract Inhibits Breast Cancer Progression Through a LncRNA-H19/MiR-675-5p Pathway | |
| IUPAC name | NA | |
| Phytochemicals’ class or type of plant extracts | Aqueous extract | |
| Source of phytochemicals or plant Extracts | Trametes robiniophila murr | |
| Geographical availability | China | |
| Plant parts | Fungi | |
| Other cancers | Breast cancer, Liver cancer, Lung cancer, Ovarian cancer, Stomach cancer | |
| Target gene or protein | H19, ATM, miR-203, miR-675-5p, MMP2, MMP9, VEGF, ERK, p65, JNK, STAT-3, CBL, ATM | |
| Gene or Protein evidence | The over-expression of H19 inhibited the cytotoxic effects of Huaier extract. In contrast, reduced H19 expression enhanced the function of Huaier extract. Huaier extract restrains the proliferative potential of endocrine-resistant breast cancer cells through increased ATM by suppressing miR-203. Upregulating miR-675-5p reversed the inhibitory effects of Huaier extract, whereas downregulating miR-675-5p sensitized breast cancer cells to the effect of Huaier extract. Additionally, western blotting showed decreased expression of MMP2, MMP9 and VEGF with the use of Huaier extract. As determined by western blot analysis, Huaier extract dose-dependently decreased the levels of phosphorylated extracellular signal-regulated kinase (ERK), transcription factor p65, c-Jun N-terminal kinase (JNK), signal transducer and activator of transcription 3 (STAT3) and the expression of vascular endothelial growth factor (VEGF). In addition, Huaier extract increased the expression of CBL protein, a direct target of miR-675-5p. | |
| Target pathways | These results show that Huaier extract synergizes with TAM to induce autophagy and apoptosis in ER-positive breast cancer cells by suppressing the AKT/mTOR pathway. Huaier aqueous extract inhibits stem-like characteristics of MCF7 breast cancer cells via inactivation of hedgehog pathway. The molecular mechanisms may be involved in the inhibition of the NF-κB pathway and c-Met expression. | |
| IC50 | NA | |
| Potency | Our data demonstrated that Huaier extract effectively inhibited the proliferation of the MCF-7, T47D and ZR-75-1 human breast cancer cell lines. These findings provided experimental evidence that Huaier extract was a promising therapeutic drug for eliminating the breast cancer stem cells. These data suggest that Huaier extract may serve as a potent anti-angiogenic and antitumor agent. Huaier aqueous extract inhibits proliferation of breast cancer cells by inducing apoptosis. | |
| Cell line/ mice model | T47D, ZR-75-1, MCF-7, MDA-MB-231, M7-TR, M7-FR, RAW264.7 murine macrophage, 4T1 tumor bearing mice | |
| Additional information | In addition, treatment with Huaier extract decreased the motility and tube formation of HUVECs in a dose-dependent manner in vitro. In addition, Huaier extract reduced the number of cells expressing CD44+/CD24- and decreased the level of stem cell markers (OCT-4, NESTIN, and NANOG). In addition, Huaier extract increased the expression of CBL protein, a direct target of miR-675-5p. In addition, breast cancer patients with Huaier granule treatment had higher KPS scores and less emotional symptoms. Collectively, our results indicated that Huaier extract increased the antitumor effect of paclitaxel therapy in breast cancer cells. Our study revealed a novel mechanism of the anti-tumor effect of Huaier extract which inhibited angiogenesis by targeting TAMs. Our study provides evidence that Huaier extract is a novel estrogen receptor modulator and is a promising drug for the prevention and treatment of ERα-positive human breast cancers. | |
| PubChem ID | NA | |
| Additional PMIDs | 20718753 22895629 23686317 25077927 25826742 26831282 27245026 27027343 29145193 28779143 29039556 30276759 | |
| Additional sources of information | NA | |
| Safety | NA |