| Properties | Information | |
|---|---|---|
| PhytoCAT-ID | PhytoCAT-1223 | |
| Phytochemical name or plant extracts | Dentatin (DTN) | |
| PMID | 23178663 | |
| Literature evidence | Acridine orange/propidium iodide staining was used to detect cells in early apoptosis and high content screening (HCS) to observe nuclear condensation, cell permeability, mitochondrial membrane potential (MMP) and cytochrome c release. | |
| IUPAC name | 5-methoxy-2,2-dimethyl-10-(2-methylbut-3-en-2-yl)pyrano[3,2-g]chromen-8-one | |
| Phytochemicals’ class or type of plant extracts | Coumarin | |
| Source of phytochemicals or plant Extracts | Clausena excavata Burm | |
| Geographical availability | Assam, Bangladesh, Borneo, Cambodia, China South-Central, China Southeast, East Himalaya, Hainan, India, Jawa, Laos, Lesser Sunda Is., Malaya, Myanmar, Nepal, New Guinea, Philippines, Sulawesi, Sumatera, Taiwan, Thailand, Vietnam | |
| Plant parts | Root and Stem | |
| Other cancers | Breast cancer | |
| Target gene or protein | Bcl-2, Bax, Caspase 9, Caspase 3, Caspase 7, cyt-c | |
| Gene or Protein evidence | Treatment of MCF-7 cells with DTN encouraged apoptosis with cell death-transducing signals that reduced MMP by down-regulation of Bcl-2 and up-regulation of Bax, triggering cytochrome c release from the mitochondria to the cytosol. The released cytochrome c triggered the activation of caspase 9 followed by the executioner caspase 3/7. | |
| Target pathways | NF-κB signalling pathway | |
| IC50 | 6.13±0.01 µg/ml against MCF-7 | |
| Potency | DTN treatment significantly arrested MCF-7 cells at the G0/G1 phase (p<0.05) and ROS was significantly elevated. | |
| Cell line/ mice model | MCF-7 | |
| Additional information | NA | |
| PubChem ID | 342801 | |
| Additional PMIDs | NA | |
| Additional sources of information | https://powo.science.kew.org/taxon/urn:lsid:ipni.org:names:772128-1 | |
| Safety | DTN inhibited the MCF-7 cells with an IC50 of 6.1370.01 ?g/ml (18.80 ?M), whereas the IC50 for the MCF-10A cells was 23.1671.26 ?g/ml (71.04 ?M). |