| Properties | Information | |
|---|---|---|
| PhytoCAT-ID | PhytoCAT-1907 | |
| Phytochemical name or plant extracts | Annurca apple polyphenol extract (APE) | |
| PMID | 28766690 | |
| Literature evidence | Altogether our data indicate that APE, at elevated concentrations, acts as a potent pro-oxidant and antiproliferative agent able to downregulate ERK1/2 pathway leading to cell cycle inhibition and apoptosis and provides a rationale for its potential use in the development of novel therapeutics towards breast cancer. | |
| IUPAC name | NA | |
| Phytochemicals’ class or type of plant extracts | Polyphenol extract | |
| Source of phytochemicals or plant Extracts | Malus pumila Miller cv Annurca | |
| Geographical availability | Afghanistan, Kazakhstan, Kirgizstan, Pakistan, Tadzhikistan, Uzbekistan, Xinjiang | |
| Plant parts | Fruits | |
| Other cancers | Breast cancer | |
| Target gene or protein | p53, p21, Cyclin D1, ERK1/2 phosphorylation, Bax, Bcl-2 , Caspase 9,Caspase 6, Caspase 7, PARP | |
| Gene or Protein evidence | Immunoblot analysis demonstrated that APE treatment increases the levels of p53 and p21, downregulates the expression of the cell cycle regulatory protein cyclin D1, and inhibits ERK1/2 phosphorylation. Moreover, APE treatment caused a marked increase of pro-apoptotic Bax/Bcl-2 ratio paralleled by caspase-9, -6, -7, and poly(ADP ribose) polymerase cleavage. | |
| Target pathways | ERK1/2 pathway | |
| IC50 | In contrast, a prolonged incubation of 48 h resulted in a severe loss of cell viability that decreases to 43.5%, a value near the IC50, in the presence of APE 500 µM EqC. | |
| Potency | Altogether our data indicate that APE, at elevated concentrations, acts as a potent pro-oxidant and antiproliferative agent able to downregulate ERK1/2 pathway leading to cell cycle inhibition and apoptosis and provides a rationale for its potential use in the development of novel therapeutics towards breast cancer. | |
| Cell line/ mice model | MCF-7 | |
| Additional information | Results showed that at 500 µM catechin equivalent (EqC) APE acts as a pro-oxidant increasing thiobarbituric acid-reactive species cell content of approximately 6-fold more than the untreated cells. | |
| PubChem ID | NA | |
| Additional PMIDs | NA | |
| Additional sources of information | https://powo.science.kew.org/taxon/urn:lsid:ipni.org:names:726372-1 | |
| Safety | NA |