| Properties | Information | |
|---|---|---|
| PhytoCAT-ID | PhytoCAT-1274 | |
| Phytochemical name or plant extracts | Anastatica hierochuntica extracts | |
| PMID | 32792843 | |
| Literature evidence | Breast cancer therapy using anticancer bioactive compounds derived from natural products as adjuvant treatment has gained recognition due to expensive and toxic conventional chemotherapeutic drugs. | |
| IUPAC name | NA | |
| Phytochemicals’ class or type of plant extracts | Aqueous and Methanolic extract | |
| Source of phytochemicals or plant Extracts | Anastatica hierochuntica | |
| Geographical availability | Algeria, Canary Is., Egypt, Eritrea, Gulf States, Iran, Iraq, Kuwait, Lebanon-Syria, Libya, Mali, Mauritania, Morocco, Oman, Pakistan, Palestine, Saudi Arabia, Sudan, Tunisia, Western Sahara, Yemen | |
| Plant parts | Leaves, Seeds and Stem | |
| Other cancers | Breast cancer | |
| Target gene or protein | Tp53, CDKN1A, Caspase 3, Caspase 9, Bax | |
| Gene or Protein evidence | The induction of apoptosis was observed in all the methanolic and aqueous-treated MCF-7 cells. The cell death process was confirmed by the visualization of DNA fragmentation and cleavage of the intrinsic apoptotic pathways, caspase-9 and caspase-3, the key enzyme causing apoptosis hallmarks. In addition, the most pro-apoptotic extracts, ASD and ML, up-regulated the expression of pro-apoptotic Bax, tumor suppressor TP53 genes and the cyclin inhibitor CDKN1A gene. | |
| Target pathways | Methanolic (MSD, MST, ML) and aqueous (ASD, AST, AL) extracts of A. hierochuntica’s stem, seeds and leaves inhibited the viability of MCF-7, cell line through the induction of apoptosis involving the intrinsic-dependent apoptotic pathway, A. hierochuntica initiates apoptosis through the mitochondria-dependent intrinsic pathway. | |
| IC50 | Methanolic seed extract - 458.899 µg/ml against MCF-7 Methanolic stem extract - 539.619 µg/ml against MCF-7 Methanolic leaves extract - 293.268 µg/ml against MCF-7 Aqueous seed extract -282.501 µg/ml against MCF-7 Aqueous stem extract - 448.054 µg/ml against MCF-7 Aqueous leaves extract - 375.226 µg/ml against MCF-7 | |
| Potency | In conclusion, of the aqueous and methanolic extracts of A. hierochuntica plant parts exerting antiproliferative effects through the induction of apoptosis in breast cancer MCF-7 cells, ASD and ML extracts were the most promising natural-based drugs for the treatment of breast cancer. | |
| Cell line/ mice model | MCF-7 | |
| Additional information | We assessed the anticancer potential of different parts of A. hierochuntica (seeds, stems and leaves) and explored their mechanisms of action using the human breast cancer cell line, MCF-7. Currently, we investigated the antiproliferative effects of methanolic (MSD, MST, ML) and aqueous (ASD, AST, AL) extracts of A. hierochuntica plant parts on the MCF-7 cells using cell viability assays. | |
| PubChem ID | NA | |
| Additional PMIDs | NA | |
| Additional sources of information | https://powo.science.kew.org/taxon/urn:lsid:ipni.org:names:277878-1 | |
| Safety | NA |