| Properties | Information | |
|---|---|---|
| PhytoCAT-ID | PhytoCAT-585 | |
| Phytochemical name or plant extracts | 7α-hydroxy-β-sitosterol | |
| PMID | 22997533 | |
| Literature evidence | In continuation of our interest towards the elucidation of apoptotic pathways of cytotoxic phytocompounds, we have embarked upon a study on the anticancer effects of 7α-hydroxy-β-sitosterol (CT1), a rare natural phytosterol oxide isolated from Chisocheton tomentosus. | |
| IUPAC name | NA | |
| Phytochemicals’ class or type of plant extracts | Phytosterols | |
| Source of phytochemicals or plant Extracts | Chisocheton tomentosus | |
| Geographical availability | Malaya, Thailand | |
| Plant parts | Bark | |
| Other cancers | Breast cancer, Cervical cancer, Oral cancer, Liver cancer | |
| Target gene or protein | Bax, Bcl-2, procaspase 9, procaspase 3, ERK1/2, FasL, Bim | |
| Gene or Protein evidence | CT1 was also found to increase proapoptotic Bax protein levels, while decreasing anti-apoptotic Bcl-2 protein levels, suggesting the involvement of the intrinsic pathway. Reduced levels of initiator procaspase-9 and executioner procaspase-3 were also observed following CT1 exposure, confirming the involvement of cytochrome c-mediated apoptosis via the mitochondrial pathway. CT1 Reduces ERK1/2, FasL, Bcl-2, and Bim Protein Levels | |
| Target pathways | ERK1/2 signaling pathway | |
| IC50 | 38.2 ± 3.2 μM for 12h against MCF-7 28.6 ± 4.1 μM for 18h against MCF-7 16.0 ± 3.6 μM for 24h against MCF-7 | |
| Potency | These results demonstrated the cytotoxic and apoptotic ability of 7α-hydroxy-β-sitosterol and suggest its potential anti-cancer use particularly on breast adenocarcinoma cells. | |
| Cell line/ mice model | MCF-7, HMEC, HSC-4, HepG2 | |
| Additional information | Our results showed that cell death in MCF-7 breast tumor cells was achieved through the induction of apoptosis via downregulation of the ERK1/2 signaling pathway. | |
| PubChem ID | NA | |
| Additional PMIDs | NA | |
| Additional sources of information | https://powo.science.kew.org/taxon/urn:lsid:ipni.org:names:577954-1 | |
| Safety | NA |